Rapid, ELISA-based measurement of protein tyrosine kinase activity using the K-LISA™ Kit

Rapid, ELISA-based measurement of protein tyrosine kinase activity using the K-LISATM Kit

The K-LISATM PTK Screening Kit is a colorimetric kit in a 96-well plate format, which provides a rapid and sensitive method for detection of protein tyrosine kinase (PTK) activity in purified or partially purified kinase preparations, cell lysates and tissue extracts. The kit features a multiplex array of three synthetic, random polypeptide substrates of glutamic acid, alanine and tyrosine, whi...

Rapid Activation of Protein Tyrosine Kinase

Phosphorylation of Staphylococcus aureus Protein-Tyrosine Kinase Affects the Function of Glucokinase and Biofilm Formation

Background: When Staphylococcus aureus is grown in the presence of high concentration of external glucose, this sugar is phosphorylated by glucokinase (glkA) to form glucose-6-phosphate. This product subsequently enters into anabolic phase, which favors biofilm formation. The presence of ROK (repressor protein, open reading frame, sugar kinase) motif, phosphate-1 and -2 sites, and tyrosine kina...

Inhibition of c-kit receptor tyrosine kinase activity by STI 571, a selective tyrosine kinase inhibitor.

STI 571 (formerly known as CGP 57148B) is a known inhibitor of the c-abl, bcr-abl, and platelet-derived growth-factor receptor (PDGFR) tyrosine kinases. This compound is being evaluated in clinical trials for the treatment of chronic myelogenous leukemia. We sought to extend the activity profile of STI 571 by testing its ability to inhibit the tyrosine kinase activity of c-kit, a receptor struc...

Using Recombinant Chlamydia Major Outer Membrane Protein (MOMP) in ELISA Diagnostic Kit

Chlamydia trachomatis is one of the main causes of Sexually Transmitted Diseases (STDs) such as  prostatitis and epididymitis in men and cervicitis, endometriosis, vaginitis and ureogenital tract infections in women.  Serological tests with sensitivities related to specific antigens are commonly used as  routine laboratory tests for diagnosis of Chlamydia. In this research the Chlamydia Major O...